Determination of a-alkyl-a-amino acids and a-amino alcohols by chiral phase capillary gas chromatography and reversed phase HPLC
Summary
Type: HPLC Application #: 105390
Catalog#: 720081.40 - MN HPLC Column, Nucleosil Chiral-1, 120A, 5um, 4x250mm
Detection: UV, 245nm Conditions: eluent: 5% acetonitrile in 1.0mM copper acetate buffer adjusted to pH 5.6 with HCl flow rate 1ml/min pressure 7MPa Authors: Brückner, H. et al. Source: J. Chromatogr. 395 (1987) 569590
Details
Legend: Fig. 1. Separation of selected D,L-a-amino acids by ligand exchange HPLC
a) D,L-isovaline (IVA)
b) D,L-a-methyltyrosine (a-Me-Tyr)
c) D,L-a-methylleucine (a-Me-Leu)
50nmol in 20µl each injected.